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GrainGenes Probe Report: WMC106

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC106
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7e6 Donor: Somers, Agriculture Canada Trimmed to insert at 5' end. 3' end not located. (CA)21 147 to 188. Primer dimer with these primers. Redesign primers. Bases 60 to 144 show homology to 25f8 bases 13 to 97 (85 in longest run, maxScore = 75).
Type	PCR SSR
PCR primers	Forward cTAATTggATgcAggTgAcA at 2 length 20 bases, Tm 59 Reverse ccccAcTTcAAcTcgcTATg at 299 length 20 bases, Tm 62
SSR size	318 bases expected, Chinese Spring
Amplification Conditions	tested 51C anneal, no product;
Sequence	WMC106
Source Species	Triticum aestivum