GrainGenes Probe Report: WMC105
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC105 [ Marker Report ]
Locus
Xwmc105-6B Xwmc105 Xwmc105y wmc105 wmc105-6B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7f5 Donor: Somers, Agriculture Canada Trimmed to insert (Rsa1 at 5' end). (GT)14 77 to 104. (2 loci - both polymorphic?)
Type
PCR SSR
PCR primers
Forward AATgTcATgcgTgTAgTAgccA at 6 length 22 bases, Tm 62
Reverse AAgcgcAcTTAAcAgAAgAggg at 176 length 22 bases, Tm 64
SSR size
192 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC105
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC105
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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