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GrainGenes Probe Report: WMC103

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC103  [ Marker Report ]
Locus	Xwmc103.1 Xwmc103.2
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7h4 Donor: Somers, Agriculture Canada Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 131 bases located on 6A, 290 bases located on 6B, 294 bases located on 2A Trimmed to insert at 5' end, 3' end not located. (GCC)7 322 to 342. 1 polymophic locus from 2 or 3)
Type	PCR SSR
PCR primers	Forward AgcgTTgTcTAggTTTAggTTg at 94 length 22 bases, Tm 59 Reverse TAgTAgcTAgccAgATggATTg at 365 length 22 bases, Tm 58
SSR size	293 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC103
Source Species	Triticum aestivum