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GrainGenes Probe Report: WMC100

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC100
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d3 Donor: Somers, Agriculture Canada Trimmed to insert at both ends (Rsa1 at 5' end). (GT)32 218 to 281.
Type	PCR SSR
PCR primers	Forward gAgccAAATTTgcTgTTTccT at 195 length 21 bases, Tm 62 Reverse gcATccTcTTAAcTgcTgTgA at 399 length 21 bases, Tm 60
SSR size	225 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC100
Source Species	Triticum aestivum