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GrainGenes Probe Report: WMC97

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC97  [ Marker Report ]
Locus	Xwmc97-5D Xwmc97 wmc97
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7f2 Donor: Somers, Agriculture Canada Trimmed to insert at both ends (Rsa1 at 3'). (GT)26 176 to 227. Tight fit for primers. Complex band pattern. Bases 50 to 162 show homology to 2g6.1 bases 75 to 187 (complement) (113 in longest run, maxScore = 98). True duplicate with 2g6.1, 7f2 has priority.
Type	PCR SSR
PCR primers	Forward gTccATATATgcAAggAgTc at 59 length 20 bases, Tm 54 Reverse gTAcTcTATcgcAAAAcAcA at 223 length 20 bases, Tm 53
SSR size	184 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC97
Source Species	Triticum aestivum