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GrainGenes Probe Report: WMC96

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC96  [ Marker Report ]
Locus	Xwmc96-4A [ Show all 16 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d2 Donor: Somers, Agriculture Canada Trimmed to insert at both ends. (CA)29 177 to 234. (1 locus polymophic out of 3)
Type	PCR SSR
PCR primers	Forward TAgcAgccATgcTTAgcATcAA at 28 length 22 bases, Tm 65 Reverse gTTTcAgTcTTTcAcgAAcAcg at 286 length 22 bases, Tm 62
SSR size	280 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC96
Source Species	Triticum aestivum