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GrainGenes Probe Report: WMC95

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC95  [ Marker Report ]
Locus	Xwmc95-1A [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7f1 Donor: Somers, Agriculture Canada Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 178-211 bases located on 1A, 205 bases located on 6B Trimmed to insert at both ends. (GT)18 179 to 214. A struggle to fit primers. (3 polymorphic loci?) Bases 83 to 173 show homology to 19d5 bases 1 to 91 (91 in longest run, maxScore = 75).
Type	PCR SSR
PCR primers	Forward gTTTTTgTgATcccgggTTT at 53 length 20 bases, Tm 63 Reverse cATgcgTcAgTTcAAgTTTT at 229 length 20 bases, Tm 58
SSR size	196 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC95
Source Species	Triticum aestivum