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GrainGenes Probe Report: WMC93

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC93  [ Marker Report ]
Locus	Xwmc93-1A [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6h12 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 146 bases located on 1A, 155 bases located on 1D AGR144. Multiple inserts (2) joined by linkers at 331-344. Trimmed at both ends to insert. (GT)8 31 to 46, (GT)12 49 to 72, (GT)24 456 to 503. Primers here are for second insert, cannot design for first. (2 polymorphic loci?) Bases 1 to 328 show homology to 12e6.1 bases 1 to 328 (328 in longest run, maxScore = 244). Bases 347 to 638 show homology to 12e6.2 bases 3 to 294 (292 in longest run, maxScore = 205).
Type	PCR SSR
PCR primers	Forward AcAAcTTgcTgcAAAgTTgAcg at 428 length 22 bases, Tm 64 Reverse ccAAcTgAgcTgAgcAAcgAAT at 555 length 22 bases, Tm 66
SSR size	149 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC93
Source Species	Triticum aestivum