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GrainGenes Probe Report: WMC92

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC92
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6e12 Donor: Devaux, Florimond Desprez AGR141. Trimmed to Rsa1 at 3' . (CA)15 263 to 292, (CT)9 245 to 262. Related to 7a3 (3rd insert), 8d10, 5a10, 11a3, 9g4, 5g2. Smear on agarose. Bases 389 to 420 show homology to 10a9.1 bases 219 to 250 (32 in longest run, maxScore = 91), 6e12 has precedence.
Type	PCR SSR
PCR primers	Forward TgTATTTAcgAgcATcgAccAA at 206 length 22 bases, Tm 62 Reverse AATAATTcAAcgggTcccAcTT at 306 length 22 bases, Tm 62
SSR size	122 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, smear;
Sequence	WMC92
Source Species	Triticum aestivum