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GrainGenes Probe Report: WMC91

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC91
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6d12 Donor: Devaux, Florimond Desprez AGR140. Trimmed to insert. (CT)23 105 to 150. Bases 154-328 (ie. 3' side of microsatellite) match part of 3g4. Bases 105-253 (ie. 3' side of microsatellite) match part of 3A8 (complement). Bases 4-150 (ie. 5' side of microsatellite) match part of 13C3 (complement). Bases 169 to 230 show homology to 14a10 bases 224 to 285 (complement) (62 in longest run, maxScore = 126).
Type	PCR SSR
PCR primers	Forward TcTcggccTcTccAgATcTAAA at 66 length 22 bases, Tm 64 Reverse AggATccggcTggATcTgTgAc at 162 length 22 bases, Tm 69
SSR size	118 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; tested 51C anneal, no product;
Sequence	WMC91
Source Species	Triticum aestivum