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GrainGenes Probe Report: WMC88

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC88
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g11 Donor: Devaux, Florimond Desprez AGR135. Trimmed to insert at 3'. (CA) complex microsatellite. Amplifies many smaller fragments, some are polymorphic - (a bit RAPD like) redesign primers? Bases 194 to 251 show homology to 15h3.2 bases 104 to 161 (complement) (58 in longest run, maxScore = 55).
Type	PCR SSR
PCR primers	Forward TgcAAcTAggggAcTAcAAA at 14 length 20 bases, Tm 58 Reverse AgTTgcATgTcAAgcATcAg at 418 length 20 bases, Tm 59
SSR size	424 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC88
Source Species	Triticum aestivum