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GrainGenes Probe Report: WMC86

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC86
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6b10 Donor: Devaux, Florimond Desprez AGR122 No overlap between forward and reverse sequences. Trimmed to insert at 3' end. (GT)12 355 to 378, (GT)11 630 to 651, (GT)11 662 to 683, (CT)8 339 to 354. Polymorphic though maybe best to redesign primers to get smaller PCR. Bases 169-231 match part of 8g4, forward primer outside this region. Bases 187 to 231 show homology to 21g9 bases 178 to 222 (complement) (45 in longest run, maxScore = 127).
Type	PCR SSR
PCR primers	Forward cAcTggggTgcgcATggAgATT at 80 length 22 bases, Tm 72 Reverse gggAccggATcgAgAgAAcAAg at 388 length 22 bases, Tm 68
SSR size	330 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC86
Source Species	Triticum aestivum