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GrainGenes Probe Report: WMC85

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC85  [ Marker Report ]
Locus	Xwmc85-1B [ Show all 14 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g9 Donor: Devaux, Florimond Desprez AGR119. Trimmed to insert at 3' end(CA)8 153 to 168, (CA)18 184 to 219. Products a little weak at 61C, try lower anneal?
Type	PCR SSR
PCR primers	Forward ggAgTAAgAgAAAcATgccgAA at 27 length 22 bases, Tm 62 Reverse gTgcATgcATgAgAATAggAAc at 233 length 22 bases, Tm 61
SSR size	228 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC85
Source Species	Triticum aestivum