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GrainGenes Probe Report: WMC83

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC83  [ Marker Report ]
Locus	Xwmc83-7A [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6f8 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 166 bases located on 7A AGR110. Trimmed to insert.(GT)28 32 to 87. (4 polymorphic loci?).
Type	PCR SSR
PCR primers	Forward TggAggAAAcAcAATggATgcc at 7 length 22 bases, Tm 68 Reverse gAgTATcgccgAcgAAAgggAA at 145 length 22 bases, Tm 68
SSR size	160 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC83
Source Species	Triticum aestivum