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GrainGenes Probe Report: WMC82

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC82
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6d8 Donor: Devaux, Florimond Desprez AGR108. Trimmed to Rsa1 at 3' end.(CA)17 348 to 381. Redesign primers? Duplicate of 3C10. Bases 196 to 221 show homology to 12c6 bases 207 to 232 (complement) (26 in longest run, maxScore = 69).
Type	PCR SSR
PCR primers	Forward gcAAAcAgcccATgAcATAAAT at 39 length 22 bases, Tm 63 Reverse ccTgAgTTTTcgcATAAAccAA at 383 length 22 bases, Tm 63
SSR size	366 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; tested 51C anneal, no product;
Sequence	WMC82
Source Species	Triticum aestivum