Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC81

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC81
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6b8 Donor: Devaux, Florimond Desprez AGR106. Trimmed to Rsa fragment. (CA)8 93 to 108, (CA)20 119 to 158.
Type	PCR SSR
PCR primers	Forward AcAAcAgTcTgcTccAgccAAg at 3 length 22 bases, Tm 66 Reverse cgcAcTcgcATgATTTTccTAc at 169 length 22 bases, Tm 66
SSR size	188 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product;
Sequence	WMC81
Source Species	Triticum aestivum