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GrainGenes Probe Report: WMC80

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC80
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6a8 Donor: Devaux, Florimond Desprez AGR105. Multiple inserts (2) joined buy linkers at 311-324. End of second insert trimmed to Rsa1(CA)14 159 to 186, (CA)8 211 to 226, (CAA)14 455 to 496, (CAA)10 524 to 553, (CT)9 141 to 158. Primers are for second insert containing (CAA) microsatellite. First insert Microsatellite at 3' has similar core to 4g10. First insert matches 4d4 (complement). Band is too big (~400 bases)
Type	PCR SSR
PCR primers	Forward gTcATccATTAAATgcAgcg at 401 length 20 bases, Tm 61.0 Reverse gTcATTTcggAcAAATcAgg at 604 length 20 bases, Tm 60.0
SSR size	223 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product;
Sequence	WMC80
Source Species	Triticum aestivum