Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC79

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC79  [ Marker Report ]
Locus	Xwmc079-4A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6h7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 4D, 150 bases located on 4A AGR104. Trimmed to insert. Complex microsatellite (TC)(CA)104-164. 5' side of microsatellite has a match to 2e10 etc.
Type	PCR SSR
PCR primers	Forward cATcAATgcATATggcTgAAAT at 55 length 22 bases, Tm 61 Reverse AAAAgTTgTcATgAgcgAAgAA at 185 length 22 bases, Tm 61
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC79
Source Species	Triticum aestivum