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GrainGenes Probe Report: WMC78

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC78
Locus	Xwmc78 Xwmc78-3B wmc78
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6f7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 265 bases located on 3B AGR102. Trimmed to insert. (CA)18 158 to 193. (2 loci - 1 polymorph) Bases 158 to 282 show homology to 2a3 bases 183 to 307 (125 in longest run, maxScore = 79). True duplicate with 2a3, 6f7 has priority.
Type	PCR SSR
PCR primers	Forward AgTAAATccTcccTTcggcTTc at 42 length 22 bases, Tm 64 Reverse AgcTTcTTTgcTAgTccgTTgc at 261 length 22 bases, Tm 64
SSR size	241 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC78
Source Species	Triticum aestivum