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GrainGenes Probe Report: WMC77

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC77
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6e7 Donor: Devaux, Florimond Desprez AGR101. Trimmed to insert at 3'.(GT)8 147 to 162, (GT)8 211 to 226. Software stuggled to find these primers. Bases 114-205 (across and 3' side of microsatellite ) match part of 3h1.
Type	PCR SSR
PCR primers	Forward TgTgTgTTTcgTTgTggccccc at 78 length 22 bases, Tm 73 Reverse gcTAgTTgcgAgTTTgATggTc at 257 length 22 bases, Tm 63
SSR size	201 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC77
Source Species	Triticum aestivum