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GrainGenes Probe Report: WMC76

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC76  [ Marker Report ]
Locus	Xwmc76-7B Xwmc76 wmc76-7B wmc76
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6d7 Donor: Devaux, Florimond Desprez AGR100. Trimmed to insert at 3'(GT)19 140 to 177. (3 polymorphic loci?)
Type	PCR SSR
PCR primers	Forward cTTcAgAgccTcTTTcTcTAcA at 97 length 22 bases, Tm 57 Reverse cTgcTTcAcTTgcTgATcTTTg at 331 length 22 bases, Tm 62
SSR size	256 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC76
Source Species	Triticum aestivum