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GrainGenes Probe Report: WMC75

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC75  [ Marker Report ]
Locus	Xwmc75 Xwmc75-5B wmc75 wmc75-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6c7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5B AGR99. Fragment trimmed to insert(GT)13 117 to 142. Matches 3a6.
Type	PCR SSR
PCR primers	Forward gTccgccgcAcAcATcTTAcTA at 51 length 22 bases, Tm 66 Reverse gTTTgATccTgcgAcTcccTTg at 235 length 22 bases, Tm 67
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC75
Source Species	Triticum aestivum