GrainGenes Probe Report: WMC73
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC73 [ Marker Report ]
Locus
Xwmc73-5B Xwmc73 Xwmc73b wmc73 wmc73-5B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1h12 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 173 bases located on 5B, 192 bases located on 5B, 579 bases located on 5B (CA)9 53 to 70. (3 out of 4 loci polymorphic?)
Type
PCR SSR
PCR primers
Forward TTgTgcAccgcAcTTAcgTcTc at 7 length 22 bases, Tm 67.0
Reverse AcAcccggTcTccgATccTTAg at 174 length 22 bases, Tm 67.0
SSR size
189 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC73
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC73
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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