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GrainGenes Probe Report: WMC72

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC72
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1g12 Donor: Waugh, SCRI (CA)18 217 to 252, also CT 197-216. (2-3 polymorphic loci). Highly related to 2e10, 4c11, 6d5
Type	PCR SSR
PCR primers	Forward TggcTgAAAgATgTATTAcgAg at 161 length 22 bases, Tm 59.0 Reverse AATTcAAgggTcccAcTTATgT at 266 length 22 bases, Tm 60.0
SSR size	127 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC72
Source Species	Triticum aestivum