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GrainGenes Probe Report: WMC71

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC71
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1f12 Donor: Waugh, SCRI (GA)9 354 to 371, (GT)8 194 to 209, (GT)13 312 to 337, (GT)10 391 to 410. First 120 bases have ssome homology to 1g1 family.
Type	PCR SSR
PCR primers	Forward TAcgggTAAggTTggATgAAgg at 103 length 22 bases, Tm 64 Reverse cTTAAATTAcATccgccAcAgg at 420 length 22 bases, Tm 62
SSR size	339 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC71
Source Species	Triticum aestivum