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GrainGenes Probe Report: WMC69

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC69  [ Marker Report ]
Locus	Xwmc69.2 Xwmc69.1 wmc69
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1b12 Donor: Waugh, SCRI (CT)13 352 to 377. (2-3 polymorphic loci?).
Type	PCR SSR
PCR primers	Forward TcTgcTcTTAgcTAggAcgATg at 291 length 22 bases, Tm 61.0 Reverse cAgATgTTccccTTcAAAATgg at 500 length 22 bases, Tm 64.0
SSR size	231 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC69
Source Species	Triticum aestivum