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GrainGenes Probe Report: WMC66

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC66
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1a11 Donor: Waugh, SCRI (CA)11 24 to 45, (CT)7T(CT)9 196 to 229, only possible to design primers to second microsatellite
Type	PCR SSR
PCR primers	Forward ccTATcTAgTAggccTcTcgcA at 154 length 22 bases, Tm 62 Reverse cccTTcgAATTggTTgTTgTgg at 313 length 22 bases, Tm 67
SSR size	181 bases expected, Chinese Spring
Sequence	WMC66
Source Species	Triticum aestivum