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GrainGenes Probe Report: WMC64

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC64
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1c10 Donor: Waugh, SCRI (CA)10 141 to 160. 1st hundred bases are related to 1F11. Froward primer is fro least homologous part of the alignment. Only improvement would be to move it a few bases 5'.
Type	PCR SSR
PCR primers	Forward AcATAcAATgggATccAgcAgA at 57 length 22 bases, Tm 63 Reverse TTTgTgcATgcAAgTgccAgAA at 191 length 22 bases, Tm 69
SSR size	156 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC64
Source Species	Triticum aestivum