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GrainGenes Probe Report: WMC60

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC60
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1c9 Donor: Waugh, SCRI (CA)28 146 to 201. (2 polymorphic loci), bands a little weak.
Type	PCR SSR
PCR primers	Forward TcATAcAcAAAAgATTcAggAT at 59 length 22 bases, Tm 56 Reverse TggAgATAcAAAgAAccATAcT at 214 length 22 bases, Tm 55
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC60
Source Species	Triticum aestivum