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GrainGenes Probe Report: WMC58

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC58  [ Marker Report ]
Locus	Xwmc58-7A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1a8 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 7B, 233 bases located on 7A, 241 bases located on 7D (GCC)6 175 to 192
Type	PCR SSR
PCR primers	Forward ATcggcggTgAAgcTgATAAgT at 32 length 22 bases, Tm 66 Reverse TgAAAcAggggAATcAgAgggT at 247 length 22 bases, Tm 66
SSR size	237 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC58
Source Species	Triticum aestivum