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GrainGenes Probe Report: WMC57

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC57
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1g7 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 1B (CA)15 158 to 187. May be dominant marker.
Type	PCR SSR
PCR primers	Forward AAcTATgTgcTAcccAAcAgAc at 135 length 22 bases, Tm 58 Reverse gAcTAcgATgAcAAccccAAAT at 242 length 22 bases, Tm 62
SSR size	129 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC57
Source Species	Triticum aestivum