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GrainGenes Probe Report: WMC56

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC56
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1f7 Donor: Waugh, SCRI (CA)12 109 to 132, (CT)10 89 to 108. Bases 49 to 68 show homology to 12c10.1 bases 43 to 62 (20 in longest run, maxScore = 32).
Type	PCR SSR
PCR primers	Forward TTcgTccccATcccAgAcTAAc at 29 length 22 bases, Tm 66 Reverse AgAgATATcgATTcgggggTgc at 197 length 22 bases, Tm 67
SSR size	190 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC56
Source Species	Triticum aestivum