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GrainGenes Probe Report: WMC55

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC55
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1e7 Donor: Waugh, SCRI (CT)9 92 to 109, expected fragment 199 bases. Bases 48 to 140 show homology to 26c3 bases 45 to 137 (complement) (93 in longest run, maxScore = 43). Duplicate with 26c3, 1e7 has priority.
Type	PCR SSR
PCR primers	Forward AcAcATcAccTcTcTATgATgAAgc at 4 length 25 bases, Tm 62 Reverse ggAATTTgTccATgTTATgcAAgTA at 178 length 25 bases, Tm 63
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC55
Source Species	Triticum aestivum