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GrainGenes Probe Report: WMC54

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC54  [ Marker Report ]
Locus	Xwmc54 wmc54
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1c7 Donor: Waugh, SCRI (GT)8 176 to 191, (GT)8 194 to 209. Bands tend to be faint.
Type	PCR SSR
PCR primers	Forward TATTgTgcAATcgcAgcATcTc at 141 length 22 bases, Tm 65 Reverse TgcgAcATTggcAAccAcTTcT at 261 length 22 bases, Tm 69
SSR size	142 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC54
Source Species	Triticum aestivum