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GrainGenes Probe Report: WMC48

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC48  [ Marker Report ]
Locus	Xwmc48-4B [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6c4 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 139 bases located on 4A, 149 bases located on 4D, 190 bases located on 4B AGR75. Trimmed to insert. (GA)9 177 to 194. Not sure if polymorphic. Contains sequence element 104-172 that matches complement of 6a12. Jorge Dubcovsky maps this to all group 4 homoeologues.
Type	PCR SSR
PCR primers	Forward gAgggTTcTgAAATgTTTTgcc at 101 length 22 bases, Tm 64 Reverse AcgTgcTAgggAggTATcTTgc at 202 length 22 bases, Tm 63
SSR size	123 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC48
Source Species	Triticum aestivum