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GrainGenes Probe Report: WMC47

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC47  [ Marker Report ]
Locus	Xwmc47-4B [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6f3 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 127 bases located on 4B, 144 bases located on 4B AGR70. Trimmed to insert at 3' end. (CA)8 86 to 101. (3 loci).
Type	PCR SSR
PCR primers	Forward gAAAcAgggTTAAccATgccAA at 30 length 22 bases, Tm 65 Reverse ATggTgcTgccAAcAAcATAcA at 149 length 22 bases, Tm 65
SSR size	141 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC47
Source Species	Triticum aestivum