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GrainGenes Probe Report: WMC46

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC46
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6d3 Donor: Dubcovsky, Buenos Aires AGR68. Multiple inserts (2) joined 227-240 by linkers. Complex microsatellite 97-198 in first insert. (>2 loci). Internal portion of second insert is duplicated in 3H12. Bases 243 to 311 show homology to 17f9 bases 15 to 83 (complement) (69 in longest run, maxScore = 58).
Type	PCR SSR
PCR primers	Forward AATccTgTTTTccATgcTcgTg at 67 length 22 bases, Tm 65 Reverse gAcgAATcgAgAgAgATggTTg at 185 length 22 bases, Tm 63
SSR size	140 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC46
Source Species	Triticum aestivum