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GrainGenes Probe Report: WMC45

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC45
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6h2 Donor: Dubcovsky, Buenos Aires AGR64. Trimmed to Rsa1 at 5'. (CT)17 144 to 177. (Several loci). 3' PRIMER IS WITHIN VECTOR SEQUENCE (LINKER DISRUPTED) - why does this PCR work?
Type	PCR SSR
PCR primers	Forward TggAgAggTggAgTggAgTggg at 119 length 22 bases, Tm 70 Reverse gAcggccAgTgAATTccTTgAg at 250 length 22 bases, Tm 67
SSR size	153 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC45
Source Species	Triticum aestivum