GrainGenes Probe Report: WMC43
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC43 [ Marker Report ]
Locus
Xwmc43-3B
[ Show all 14 ]
Synonym
Synonym HAR35_25148 HAR35_49365
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6b2 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 323 bases located on 3B, 330 bases located on 3D AGR58. Trimmed to insert at 3' end. (GT)14 429 to 456.
Type
PCR SSR
PCR primers
Forward TAgcTcAAccAccAcccTAcTg at 194 length 22 bases, Tm 63
Reverse AcTTcAAcATccAAAcTgAccg at 493 length 22 bases, Tm 63
SSR size
321 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 51C anneal, product;
Sequence
WMC43
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC43
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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