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GrainGenes Probe Report: WMC41

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC41  [ Marker Report ]
Locus	Xwmc41-2D Xwmc41 wmc41
Synonym	Synonym HAR35_28634
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6c1.2 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 115 bases located on 2D, 154 bases located on 2D, 164 bases located on 2D AGR51. Multiple inserts (2) joined 260-277. Trimmed to insert at 3' end. (GA)25 138 to 187, (GCC)7 552 to 572. Primers shown here are for second insert. Products obtained after PCR at 61C anneal, (>2 loci) - polymorphic on agarose.
Type	PCR SSR
PCR primers	Forward TcccTcTTccAAgcgcggATAg at 484 length 22 bases, Tm 70 Reverse ggAggAAgATcTcccggAgcAg at 625 length 22 bases, Tm 69
SSR size	163 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC41
Source Species	Triticum aestivum