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GrainGenes Probe Report: WMC40

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC40
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6c1.1 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 170-260 bases located on 3B, 260 bases located on 4A AGR51. Multiple inserts (2) joined 260-277. Trimmed to insert at 3' end. (GA)25 138 to 187, (GCC)7 552 to 572. Primers shown here are for first insert. Bases 79 to 102 show homology to 12e5 bases 161 to 184 (complement) (24 in longest run, maxScore = 53).
Type	PCR SSR
PCR primers	Forward TTcccTccAAAATggccAAcTg at 11 length 22 bases, Tm 69 Reverse TcTTggcTggTgcgATgAAcTg at 208 length 22 bases, Tm 70
SSR size	219 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC40
Source Species	Triticum aestivum