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GrainGenes Probe Report: WMC39

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC39
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6b1 Donor: Dubcovsky, Buenos Aires AGR50 (CA)10 311 to 330, (CT)10 291 to 310, trimmed to Rsa at 3' end. Bases 439-506 match bases 16-82 of 6B12 Bases 191 to 213 show homology to 10c5 bases 157 to 179 (23 in longest run, maxScore = 98).
Type	PCR SSR
PCR primers	Forward TccTTTccgTcTccATcccAgT at 155 length 22 bases, Tm 68 Reverse gAgTgTgcgggcgTATAAAccT at 397 length 22 bases, Tm 66
SSR size	264 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC39
Source Species	Triticum aestivum