GrainGenes Probe Report: WMC38
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC38
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6A1 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 2A, 7D, 216 bases located on 7D, 356 bases located on 7D AGR49. No overlap between forward and reverse sequences. Multiple inserts (2) joined 263-278 by linkers. (CA)25 212 to 261, (CT)13 441 to 466. Primers designed to second insert - expected fragement size does not take into account no overlap between forward and reverse. PCR products obtained of circa 1000 bases after PCR at 61C anneal. Bases670-750 have some homology to 13g1.1. Bases 104 to 121 show homology to 21c5 bases 95 to 112 (18 in longest run, maxScore = 44). Bases 152 to 199 show homology to 25f10 bases 70 to 117 (complement) (48 in longest run, maxScore = 107). Bases 611 to 637 show homology to 24a9 bases 142 to 168 (27 in longest run, maxScore = 68). Bases 201 to 257 show homology to 15f1.1 bases 201 to 257 (57 in longest run, maxScore = 143).
Type
PCR SSR
PCR primers
Forward gTAgAcgAAgTcgcggAgccTg at 284 length 22 bases, Tm 69
Reverse gcgTgAgAgAgTTAcgggcAAA at 612 length 22 bases, Tm 67
SSR size
>350 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC38
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC38
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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