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GrainGenes Probe Report: WMC37

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC37
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2b12 Donor: Wang, Utah (GT)16 101 to 132, complex microsatellite. Fragment too large at 51C. Redesign primers? (Near) duplicate of 2D7.
Type	PCR SSR
PCR primers	Forward cgcTTTgTgTgggcTgAAgAgg at 4 length 22 bases, Tm 70 Reverse gTggAcTAcAcATgAAcgggAA at 324 length 22 bases, Tm 64
SSR size	342 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product;
Sequence	WMC37
Source Species	Triticum aestivum