Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC36

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC36  [ Marker Report ]
Locus	Xwmc36 Xwmc36-1D wmc36
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2a12 Donor: Wang, Utah Several microsatellites, one (CA)36(GT)36 383 to 454. Multiple inserts (2) in this fragment (joined by linker 300-313). First insert is member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3. Primer designed against second insert 19.6.97. Bases 33 to 87 show homology to 25a1 bases 62 to 116 (complement) (55 in longest run, maxScore = 28). Duplicate with 25a1, 2a12 has priority.
Type	PCR SSR
PCR primers	Forward TTcTcTTTTccTTTcgcAcTcc at 335 length 22 bases, Tm 64 Reverse cATcAgTTgTggggTTTcTTcA at 474 length 22 bases, Tm 64
SSR size	161 bases expected, Chinese Spring
Sequence	WMC36
Source Species	Triticum aestivum