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GrainGenes Probe Report: WMC35

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC35  [ Marker Report ]
Locus	Xwmc35-2B [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2g11 Donor: Wang, Utah Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 269 bases located on 2B, 90 bases located on 2B (GT)25 113 to 162, internal repeated structure, before and after microsatellite, of around 50 bases. (>2 loci). Bases 148-235 (ie 3' side of microsatellites) match 5b8 (part). Bases 11 to 145 show homology to 14g12 bases 8 to 142 (135 in longest run, maxScore = 72).
Type	PCR SSR
PCR primers	Forward gcAgAgAggcAcAAcTAgcgAg at 4 length 22 bases, Tm 66.0 Reverse AAccTTAAgggTcTgccggAAc at 247 length 22 bases, Tm 66.0
SSR size	265 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC35
Source Species	Triticum aestivum