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GrainGenes Probe Report: WMC32

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC32
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2a11 Donor: Wang, Utah (CT)26 microsatellte(GA)26 121 to 172. Only chinese spring amplified, product ~ right size. Evidence for primer dimer.
Type	PCR SSR
PCR primers	Forward cAgAgAcgAcAgTggAcAAggg at 96 length 22 bases, Tm 66 Reverse gTccTgTgcTTTAcTTgAccgc at 182 length 22 bases, Tm 65
SSR size	108 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC32
Source Species	Triticum aestivum