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GrainGenes Probe Report: WMC30

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC30
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2e10 Donor: Wang, Utah (GA)18 Microsatellite(GA)9 69 to 86, (GA)8 89 to 104. Matches 6D5 and part of 4c1, 1g12, 6h7 (3' side of microsatellite). Bases 162 to 222 show homology to 10b5 bases 82 to 142 (complement) (61 in longest run, maxScore = 127). Duplicate with 10b5, 2e10 has priority.
Type	PCR SSR
PCR primers	Forward TTAAAAgcTgTcAcgAgcgAAg at 16 length 22 bases, Tm 63 Reverse TATgcAAgTTcAgTgTgcTATg at 199 length 22 bases, Tm 58
SSR size	205 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear;
Sequence	WMC30
Source Species	Triticum aestivum