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GrainGenes Probe Report: WMC29

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC29
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2a10 Donor: Wang, Utah Large (CA)34 microsatellite(CA)33 97 to 162. PCR products obtained at anneal 61C. Redesign primers? bases 2-130 (ie. 5' side of microsatellite) match part of 7g7, bases 139-245 ie 3' side of microsatellite match 7f11. Duplicate of 8f3.
Type	PCR SSR
PCR primers	Forward TccTTgTAAcgTgggcTggcTg at 11 length 22 bases, Tm 70 Reverse AgcccggAggTggTcgAcTgAA at 166 length 22 bases, Tm 73
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC29
Source Species	Triticum aestivum