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GrainGenes Probe Report: WMC28

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC28  [ Marker Report ]
Locus	Xwmc28-5B Xwmc28 wmc28
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2g9 Donor: Wang, Utah One small (CA) and one large (T)(CA) compound.(GT)29 132 to 189
Type	PCR SSR
PCR primers	Forward ATcAcgcATgTcTgcTATgTAT at 42 length 22 bases, Tm 59.0 Reverse ATTAgAccATgAAgAcgTgTAT at 208 length 22 bases, Tm 55.0
SSR size	188 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC28
Source Species	Triticum aestivum